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CAMP实验和reverse CAMP
发布时间：2013/06/16 微生物检验 标签：CAMPCAMP实验reversereverse CAMP浏览次数：2269 欢迎阅读！
CAMP实验协助对B群链球菌的鉴定
B群链球菌能产生CAMP因子，可促进金黄色葡萄球菌β-溶血素活性。在血琼脂平板上2种细菌交界处溶血能力增强，形成箭头状的溶血区。
试剂：血琼脂平板、金黄色葡萄球菌ATCC25923
操作：
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在血平板上ATCC25923作一横线接种，垂直处用被检菌接种一条线，2线不能相交且相距5～10mm.
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35℃孵育18～24h后观察结果.
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A CAMP positive Group B Streptococcus is streaked in the center of sheep blood agar, and Clostridium perfringens is streaked perpendicular to it. Following incubation at 37°C for 24-48 hours in anaerobic conditions, the “bow-tie” zone of enhanced hemolysis pointing towards Streptococcus agalactiae is seen. This is because of alpha toxin produced by Clostridium perfringens interacts with CAMP factor and produce synergistic hemolysis.
Procedure
Inoculate pure culture of CAMP positive S. agalactiae along the diameter of the blood agar plate by streaking.
Suspected clostridial culture is streaked at right angle to the streak of S. agalactiae (not touching to the streak of S. agalactia).
Incubate anaerobically at 37°C for 24hours.
Observe for zone of hemolysis pointing toward S. agalactiae.
reverse CAMP POS：
Corynebacterium pseudotuberculosis 假结核棒状杆菌，假结核杆菌;
cl.perfringens产气荚膜梭菌; 产气荚膜杆菌
Arcanobacterium Haemolyticum 溶血隐秘杆菌
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